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10529: Hepatitis B Virus Drug Resistance, Genotype, and BCP/Precore Mutations Print View
HVB Genotype:      
Polymerase Mutations:      
Precore Mutation:      
BCP Mutations:      

PRIMARY
1 PPT Potassium EDTA(white) 1 (0.3) mL   Frozen - 30 Day(s)/Refrigerated - 7 Day(s)/Room Temperature - 72 Hour(s)  
ALTERNATE
  Serum 1 (0.3) mL   Refrigerated - 7 Day(s)/Frozen - 30 Day(s)/Room Temperature - 72 Hour(s)  
  Plasma EDTA 1 (0.3) mL   Refrigerated - 7 Day(s)/Room Temperature - 72 Hour(s)/Frozen - 30 Day(s)  

This test is used to: 1. Identify HBV genotype (A-H) for epidemiology or prognostic purposes. 2. Detect hepatitis B virus HBV) mutations associated with resistance to antiviral agents.

Serum: Collect blood in sterile tubes with no anticoagulants; plastic
serum separator tubes (SST(R)) are recommended. Allow blood to clot
at room temperature and separate serum from cells within 2 hours of
collection. Transfer serum to sterile, plastic screw-capped, aliquot
tubes and store refrigerated or frozen. Ship frozen.

Plasma: Collect blood in sterile tubes containing EDTA anticoagulant;
either 0.15% solution v/v final EDTA K3 (standard EDTA tube) or 9 mg
spray-dried EDTA K2 (plasma preparation tube or PPT tube with plasma
separator-gel, preferred). Store whole blood at room temperature and
separate plasma from cells within 2 hours of collection. Transfer
plasma to sterile, plastic, screw-capped aliquot tubes and store at
-18 degrees or colder. If blood is collected in a PPT tube, centrifuge
preferably within 2 hours of collection as before but it is not
necessary to transfer plasma to aliquot tubes. Following
centrifugation,
a gel barrier maintains separation of plasma from cellular components
during specimen transport and storage. The PPT is plastic and hence
plasma can be stored and shipped frozen in the original tube. Avoid
repeated freezing and thawing of specimen. Ship frozen.

This test can only be performed reliably on specimens with a viral
load of at least 600 IU/mL.
Setup Schedule
Monday, Thursday

Reported (Analytical Time)
8 days

CPT Code
87912

The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.

Notes
Reject Criteria: Unspun PPT tube METHODOLOGY: Polymerase Chain Reaction (PCR), Sequencing Transport Temperature: Frozen






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