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10600: Cytomegalovirus DNA, Quantitative, Real-Time PCR Print View
Source      
CMV DNA, QN Real Time PCR RT-PCR  < 200  IU/mL 
CMV DNA, QN PCR PCR  < 2.30  Log IU/mL 

PRIMARY
1 Whole Blood EDTA 1 (0.5) mL   Room Temperature - 48 Hour(s)/Refrigerated - 8 Day(s)  
ALTERNATE
  Whole Blood ACD 1 (0.5) mL   Room Temperature - 48 Hour(s)/Refrigerated - 8 Day(s)  
  Bronchial Alveolar Lavage   Refrigerated - 8 Day(s)/Room Temperature - 48 Hour(s)/Frozen - 30 Day(s)  
  Plasma EDTA 1 (0.5) mL   Room Temperature - 48 Hour(s)/Refrigerated - 8 Day(s)/Frozen - 30 Day(s)  
  Urine   Room Temperature - 48 Hour(s)/Refrigerated - 8 Day(s)/Frozen - 30 Day(s)  
  Serum 1 (0.5) mL   Frozen - 30 Day(s)/Refrigerated - 8 Day(s)/Room Temperature - 48 Hour(s)  
  CSF 1 (0.5) mL   Room Temperature - 48 Hour(s)/Refrigerated - 8 Day(s)/Frozen - 30 Day(s)  
  Fluid Eye   Frozen - 30 Day(s)/Refrigerated - 8 Day(s)/Room Temperature - 48 Hour(s)  
  Plasma ACD 1 (0.5) mL   Room Temperature - 48 Hour(s)/Refrigerated - 8 Day(s)/Frozen - 30 Day(s)  
  Amniotic Fluid   Room Temperature - 48 Hour(s)/Refrigerated - 8 Day(s)/Frozen - 30 Day(s)  
  Plasma PPT Tube 1 (0.5) mL   Frozen - 30 Day(s)/Refrigerated - 8 Day(s)/Room Temperature - 48 Hour(s)  

In patients who are immunocompromised, CMV may cause disseminated, severe disease. CMV is also the most common cause of congenital viral infection in humans. Quantitative PCR methods may be useful in monitoring CMV replication in immunosuppressed patients or in determining the viral load of CMV in amniotic fluid. This is a quantitative molecular test, with a linear range of 200-2,000,000 IU/mL.

Whole blood: Collect whole blood in sterile tubes containing EDTA or
ACD as anticoagulant. Store refrigerated. Blood collected in tubes
containing ACD anticoagulant will yield results approximately 15%
lower when compared to EDTA tubes due to a dilution effect. Do not
freeze whole blood.

Plasma: Collect blood in sterile tubes containing EDTA or ACD as
anticoagulant or in plasma preparation tubes (PPTs). Store collected
whole blood at room temperature and separate plasma from cells within
2 hours of collection. Transfer plasma to sterile, plastic, screw-cap
tubes and store refrigerated or frozen. If blood is collected in a PPT
tube, centrifuge within 2 hours of collection and store refrigerated
or frozen. It is not necessary to transfer the plasma from a PPT tube
to aliquot tubes. Blood collected in tubes containing ACD
anticoagulant will yield results approximately 15% lower when compared
to EDTA tubes due to a dilution effect.

Serum: Collect in a red top tube (no gel) or serum separator tube.

CSF, amniotic fluid, urine, BAL, eye fluids: Collect in a sterile
container and store refrigerated or frozen.
Setup Schedule
Sunday-Saturday

Reported (Analytical Time)
Same day

CPT Code
87497

The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.

Notes
Reject Criteria: Heparinized specimens, unspun PPT tube Transport Temperature: Refrigerated Methodology: Real-Time PCR Assay Category: LDT/ASR Class I






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