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16128: RAS Mutation Analysis, Cell-Based [16128] Print View
Sample Type      
Block ID      
HRAS Mutation Analysis      
KRAS Mutation Analysis      
NRAS Mutation Analysis      

PRIMARY
1 Tissue Paraffin Block   Room Temperature - 12 Month(s)/Refrigerated - 12 Month(s)  
ALTERNATE
  Bone Marrow EDTA   Refrigerated - 72 Hour(s)/Room Temperature - 72 Hour(s)  
  Whole Blood EDTA 6 (4) mL   Refrigerated - 72 Hour(s)/Room Temperature - 72 Hour(s)  

Activating RAS mutations can be found in human malignancies with an overall frequency of 15-20%. A high incidence of RAS gene mutations has been reported in malignant tumors of the pancreas (80-90%, KRAS), in colorectal carcinomas (30-60%, KRAS), in non-melanoma skin cancer (30-50%, HRAS), in hematopoietic neoplasia of myeloid origin (18-30%, KRAS or NRAS). The presence of KRAS mutation in most tumor types is associated with adverse prognosis as well as resistance to receptor tyrosine kinase-directed targeted therapies, including against EGFR.

Submission of formalin-fixed, paraffin-embedded tissue is the
preferred sample type. Other sample types listed are acceptable
for testing. For submission of paraffin block, another preferred
specimen type, tissue source and block ID are required on the
requisition form.

A pathology report must be submitted.

Whole Blood: Follow standard whole blood collection procedures.
Collect 4-6 mLs whole blood in an EDTA tube. Record sample
type, collection time and date onto tube and requisition form.

Bone Marrow collection, the notation of sample type, collection
time and date onto the tube and requisition form is required.
Ship sample at refrigerated temperature or room temperature.
Ship immediately to maintain stability.
Setup Schedule
Tuesday-Saturday

Reported (Analytical Time)
3 days

CPT Codes
81311,81276,81275,81403

The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.

Notes
Specimen Stability: Formalin fixed paraffin embedded tissue, 5 years Room temperature and Refrigerated
Specimen Requirements:
Preferred: Formalin fixed paraffin embedded tissue
Acceptable: 6 mL (4 mL minimum) whole blood submitted in an EDTA (lavender-top) tube or 3mL (1 mL minimum) bone marrow submitted in an EDTA (lavender-top) tube
Methodology: Polymerase Chain Reaction (PCR), Sequencing
Reject Criteria: Gross hemolysis, Received frozen, Clotted,
Frozen specimens: Unacceptable






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