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16694: Hepatitis B Virus DNA, Quantitative PCR with Reflex to HBV Print View
Hepatitis B Virus DNA     IU/mL 
Hepatitis B Virus DNA     Log IU/mL 

PRIMARY
1 Plasma EDTA 4 (2.8) mL   Frozen - 30 Day(s)/Refrigerated - 6 Day(s)  
ALTERNATE
  Serum 4 (2.8) mL   Refrigerated - 6 Day(s)/Frozen - 30 Day(s)  
  PPT EDTA (White-top)   Refrigerated - 6 Day(s)/Frozen - 30 Day(s)  

Chronic carries will persist in producing detectable HBV. Patients with chronic liver disease of unknown origin most commonly have HBV that is detected by viral DNA testing. Quantitative measurement of HBV viral DNA may be used to monitor progression of disease.

Do not freeze whole blood or any samples stored in primary tube.
Freshly drawn specimens (whole blood) may be stored at 2-25 degrees
C for up to 24 hours prior to centrifugation. Separate plasma or
serum from cells within 24 hours of collection by centrifugation.
Follow manufacturer's instructions for collection tube handling.
Setup Schedule
Monday-Saturday

Reported (Analytical Time)
9-11 days

CPT Code
87517

The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.

Notes
Reflex Criteria: If Hep B Virus DNA is >=600 IU/mL then reflex to 10529
Transport Temperature: Frozen
Reject Criteria: Unspun PPT tube, Room temperature; Unacceptable
Methodology: Real-Time Polymerase Chain Reaction
Preferred Specimen: 4 mL plasma (2 mL in each of two tubes) collected in EDTA (lavender-top) tubes
Acceptable Specimens: 4 mL serum (2 mL in each of two tubes) collected in Red-top (no gel or serum separator tubes) or 4 mL (2 mL in each of two tubes) plasma collected in PPT potassium EDTA (white-top) tubes
Minimum Volume: 2.8 mL (1.4 mL in each of two tubes)






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