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16868: HIV-1 Integrase Genotype Print View
Value of Last Viral Load     copies/mL 
Date Viral Load Collected      
Raltegravir Resistance      
Elvitegravir Resistance      
Dolutegravir Resistance      

PRIMARY
1 Plasma EDTA 2 (0.6) mL   Room Temperature - 24 Hour(s)/Frozen - 42 Day(s)/Refrigerated - 6 Day(s)  
ALTERNATE
  PPT Potassium EDTA(white)   Room Temperature - 24 Hour(s)/Refrigerated - 6 Day(s)/Frozen - 42 Day(s)  
  CSF 2 (0.6) mL   Room Temperature - 24 Hour(s)/Refrigerated - 6 Day(s)/Frozen - 42 Day(s)  

The emergence of integrase drug resistance mutations has been observed in vitro and in patients experiencing virologic failure on raltegravir in clinical trials. Twenty three percent of patients receiving raltegravir in a clinical trial experienced virologic failure at 48 weeks and genotypic analysis detected raltegravir associated resistance mutations in 68% of virologic failures. This assay implifies and sequences the HIV-1 integrase gene and reports mutations at positions associated with integrase inhibitor drug resistance.

Patient Preparation: Freshly drawn specimens (whole blood) may be
stored at 2 degrees C to 25 degrees C for up to 24 hours prior to
centrifugation. Separate plasma from cells within 24 hours of
collection by centrifugation. Follow manufacturer's instructions
for collection tube handling.
CSF: Collect CSF using standard procedures. Transfer to a sterile,
leak-proof container. Ship frozen.
Setup Schedule
Monday, Friday

Reported (Analytical Time)
4 days

CPT Code
87906

The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.

Notes
METHODOLOGY: RT-PCR, Sequencing

Reject Criteria: Plasma received frozen in the plasma preparation tube (PPT); Serum; whole blood greater than 24 hours old; Frozen whole blood






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