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19704: Activated Protein C Resistance With Rflx To Factor V (leiden Print View
APC Resistance     ratio 

PRIMARY
1 Plasma Citrated 2 (1) mL   Room Temperature - 1 Hour(s)/Refrigerated - 4 Hour(s)/Frozen - 6 Month(s)  
2 Whole Blood EDTA 4 (2) mL   Refrigerated - 8 Day(s)/Frozen - 30 Day(s)/Room Temperature - 8 Day(s)  
ALTERNATE
  WB EDTA Trace Metal   Room Temperature - 8 Day(s)/Refrigerated - 8 Day(s)/Frozen - 30 Day(s)  
  Whole Blood ACD Sol B 4 (2) mL   Frozen - 30 Day(s)/Room Temperature - 8 Day(s)/Refrigerated - 8 Day(s)  

Platelet-poor plasma: Centrifuge light blue-top tube for 15 minutes at
approximately 1500 x g within 60 minutes of collection. Using a
plastic pipette, remove plasma, taking care to avoid the WBC/platelet
buffy layer and place into a plastic vial. Centrifuge a second time
and transfer platelet-poor plasma into a new plastic vial. Plasma
must be free of platelets (<10,000/mcL). Freeze immediately and
ship on dry ice.
Whole Blood: Preferred shipping temperature is frozen, but
room temperature shipping is acceptable.

Reject Criteria: Gross hemolysis
Setup Schedule
Sunday-Saturday

Reported (Analytical Time)
3 days

CPT Code
85307

The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.

Notes
If Activated Protein C-Resistance is <2.1 ratio, Factor V (Leiden) Mutation Analysis is performed at an additional charge (CPT 81241).
Specimen Requirements:
Preferred: 2 mL (1 mL minimum) frozen platelet poor citrated plasma collected in a 3.2% sodium citrate (light-blue-top) tube AND 4 mL (2 mL minimum) frozen whole blood collected in an EDTA (lavender-top) tube
Acceptable: Whole blood collected in EDTA (royal blue-top) tube or ACD solution B (yellow-top) tube
Setup Schedule: See individual assays
Transport Temperature: Frozen
Methodology: Polymerase Chain Reaction & Detection; RVVT-based Assay (Clot-based)






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