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2199: Varicella-zoster Virus Antigen Detection Print View
Specimen Source      
Varicella-zoster Virus Antigen DFA  Not detected   

PRIMARY
1 Viral Transport Media   Room Temperature - 24 Hour(s)/Refrigerated - 72 Hour(s)/Frozen - 7 Day(s) See COLLECTION INSTRUCTIONS./// 
ALTERNATE
  Swab Viral Transport   Frozen - 7 Day(s)/Refrigerated - 72 Hour(s)/Room Temperature - 24 Hour(s)  
  Sterile Container/Tube   Room Temperature - 24 Hour(s)/Refrigerated - 72 Hour(s)/Frozen - 7 Day(s)  
  Smear/Slides   Room Temperature - 48 Hour(s)/Frozen - 7 Day(s)/Refrigerated - 72 Hour(s)  
  M4 Transport Media/Swab   Frozen - 7 Day(s)/Refrigerated - 72 Hour(s)/Room Temperature - 24 Hour(s)  
  Culturette/Swab   Room Temperature - 24 Hour(s)/Refrigerated - 72 Hour(s)/Frozen - 7 Day(s)  

Aid in the diagnosis of disease caused by varicella-zoster virus (e.g., chickenpox and shingles).

1. Source of specimen is required, please include on requisition.
2. Prepare two (2) vesicular lesion smears. Scrape the base of lesion
thoroughly with a scalpel blade. Spread cellular material collected
on the edge of the blade thinly over the 6-8 mm diameter well area on
a clean microscope slide. Air dry. Fix in fresh, cold acetone (2-8C)
for 10 minutes at room temperature. Ship in slide holder
at ambient temperature.
3. Biopsy & autopsy tissues should be frozen, sectioned, and air
dried. Fix in fresh, cold acetone (2-8 C) for 10 minutes at room
temperature. Ship in slide holder at ambient temperature.

4. Vesicular lesions and biopy specimens can also be submitted in a
viral transport media or sterile saline.
5. Specimens should be collected early in the acute phase of
infection. The chance of viral recovery is best during the first 3
days after onset and is greatly reduced beyond 5 days with many
viruses.
6. Unacceptable specimens:
Wooden swabs, dry swabs, and calcium alginate.
7. Specimens received more than 72 hours from time of
draw must be frozen at -70C (not -20C) or on dry ice. Do not freeze
specimen at -20 C. Virus loses infectivity.
8. Ship specimens on cold pack or on dry ice.
9. M4 transport media (M4) and dacron-tipped swabs with plastic or
fine-wire shafts available for use with M4 are available. Please
call Client Services, 800-421-4449 to request media.
Setup Schedule
Sunday-Saturday

Reported (Analytical Time)
Next day

CPT Code
87290

The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.

Notes
Another assay available to detect Varicell-zoster virus is test code #2410 Viral Culture, as tissue culture method.






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For test information, please call Client Services at 800-421-4449.

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