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2417: AFB Culture Print View
Specimen Source      
Preliminary Culture CULTURE  By Report   
Preliminary Culture CULTURE  By Report   

PRIMARY
1 Sterile Container/Tube 1 (0.5) mL   Refrigerated - 7 Day(s)/Room Temperature - 24 Hour(s) Stain is performed by the client./// 
ALTERNATE
  Nasopharyngeal Swab   Room Temperature - 24 Hour(s)/Refrigerated - 7 Day(s)  
  Gastric Aspirate   Refrigerated - 7 Day(s)/Room Temperature - 24 Hour(s)  
  Fluid Pleural   Refrigerated - 7 Day(s)/Room Temperature - 24 Hour(s)  
  Fluid Pericardial   Room Temperature - 24 Hour(s)/Refrigerated - 7 Day(s)  
  Fluid   Room Temperature - 24 Hour(s)/Refrigerated - 7 Day(s)  
  Fluid Ascitic   Refrigerated - 7 Day(s)/Room Temperature - 24 Hour(s)  
  Culturette/Swab   Refrigerated - 7 Day(s)/Room Temperature - 24 Hour(s)  
  CSF 3 (1.5) mL   Room Temperature - 24 Hour(s)/Refrigerated - 7 Day(s)  
  Bone Marrow Heparinized   Room Temperature - 24 Hour(s)  
  Bone Marrow ACD   Room Temperature - 24 Hour(s)  
  Whole Blood Isolator Tube 10 (5) mL   Room Temperature - 24 Hour(s)  
  Whole Blood Heparin 10 (5) mL   Room Temperature - 24 Hour(s)  
  Blood Culture Bottle   Room Temperature - 24 Hour(s)  
  Whole Blood ACD 10 (5) mL   Room Temperature - 24 Hour(s)  
  Fluid Synovial   Room Temperature - 24 Hour(s)/Refrigerated - 7 Day(s)  
  Urine   Refrigerated - 7 Day(s)/Room Temperature - 24 Hour(s)  
  Tissue   Room Temperature - 72 Hour(s)/Refrigerated - 7 Day(s) Place tissue & needle biopsies in Middlebrook broth/// 
  Stool   Refrigerated - 7 Day(s)/Room Temperature - 24 Hour(s)  

The laboratory diagnosis of mycobacterial diseases depends on the detection and recovery of AFB from clinical specimens. This assay employs the use of solid media and broth-based media (MGIT) for rapid isolation of Mycobacterium species. Identifications are performed by commercial probes, HPLC, and/or conventional methods. Susceptibilities for M. tuberculosis and M. avium-intracellulare complex (MAC) are performed by the radiometric method and all others by conventional methods.

1. Collect specimen in a sterile, leakproof container.
2. For respiratory specimens, collect three sputum samples from
deep productive coughs in consecutive 8 to 24 hour intervals per CDC
guidelines (at least one should be an early morning specimen).
3. Place tissue and needle biopsies in Middlebrook broth (available
from Client Supply) or in sterile saline.
4. Culturette and nasopharyngeal swab collections may result in a
potential decline in sensitivity of Mycobacterium isolation in
culture. The yield of material is limited and, due to the
hydrophobicity of Mycobacteria, the organisms may be entrapped
within the fiber matrix and not readily transition into solution or
onto media. False negative cultures are possible, especially if few
pathogens are present.
5. If gastric aspirate cannot be shipped immediately, add 100 mg of
sodium bicarbonate as a buffer to neutralize any acid detrimental to
Mycobacteria.
6. Refrigerate all samples within 1 hour of collection time except for
whole blood samples.
7. Ship specimens on cold pack within 24 hours of collection. Whole
blood is shipped at ambient temperature.
Setup Schedule
Sunday-Saturday

Reported (Analytical Time)
2 months


Notes
1. Source of specimen is required, please include on requisition. 2. Specimens that are heavily contaminated with the normal flora may result in test delay. 3. Notify the laboratory if suspecting Mycobacterium genavense as this organism will not grow on media routinely used for Mycobacterium isolation. 4. The Mycobacterium Tuberculosis Direct (MTD), a target-amplified nucleic acid probe is available for respiratory specimens. Call technical services for details. 5. If culture is positive for Mycobacterium species, it will reflex to #2935 AFB Identification by DNA (GenProbe) or #5648 AFB Identification by HPLC at an additional charge. 6. If M. tuberculosis complex isolated, susceptibility testing will be performed and a fee will be charged. For other Mycobacterium species, susceptibility testing will be performed upon request.






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For test information, please call Client Services at 800-421-4449.

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