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2419: AFB Culture & Stain Print View
Specimen Source      
Acid-fast Bacilli Stain STAIN  No AFB Seen   
Preliminary Culture CULTURE  By Report   
Preliminary Culture CULTURE  By Report   

PRIMARY
1 Sterile Container/Tube 2 (1) mL   Refrigerated - 7 Day(s)/Room Temperature - 24 Hour(s)  
ALTERNATE
  Fluid   Room Temperature - 24 Hour(s)/Refrigerated - 7 Day(s)  
  Fluid Pericardial   Refrigerated - 7 Day(s)/Room Temperature - 24 Hour(s)  
  Fluid Peritoneal   Room Temperature - 24 Hour(s)/Refrigerated - 7 Day(s)  
  Fluid Pleural   Room Temperature - 24 Hour(s)/Refrigerated - 7 Day(s)  
  Fluid Synovial   Room Temperature - 24 Hour(s)/Refrigerated - 7 Day(s)  
  Gastric Aspirate   Refrigerated - 7 Day(s)/Room Temperature - 24 Hour(s)  
  Nasopharyngeal Swab   Room Temperature - 24 Hour(s)/Refrigerated - 7 Day(s)  
  Stool   Room Temperature - 24 Hour(s)/Refrigerated - 7 Day(s)  
  Tissue   Refrigerated - 7 Day(s)/Room Temperature - 72 Hour(s) Place tissue & needle biopsies in Middlebrook broth/// 
  Urine   Refrigerated - 7 Day(s)/Room Temperature - 24 Hour(s)  
  Whole Blood ACD 10 (5) mL   Room Temperature - 24 Hour(s)  
  Blood Culture Bottle   Room Temperature - 24 Hour(s)  
  Whole Blood Heparin 10 (5) mL   Room Temperature - 24 Hour(s)  
  Whole Blood Isolator Tube 10 (5) mL   Room Temperature - 24 Hour(s)  
  Bone Marrow ACD   Room Temperature - 24 Hour(s)  
  Bone Marrow Heparinized   Room Temperature - 24 Hour(s)  
  CSF 3 (1.5) mL   Refrigerated - 7 Day(s)/Room Temperature - 24 Hour(s)  
  Culturette/Swab   Refrigerated - 7 Day(s)/Room Temperature - 24 Hour(s)  
  Fluid Ascitic   Refrigerated - 7 Day(s)/Room Temperature - 24 Hour(s)  

The laboratory diagnosis of mycobacterial diseases depends on the detection and recovery of AFB from clinical specimens. This assay employs the use of solid media and broth based media (MGIT) for rapid isolation of Mycobacterium species. Identifications are performed by commercial probes, HPLC, and/or conventional methods. Susceptibilities for M. tuberculosis and M. avium-intracellulare complex (MAC) are performed by the radiometric method and all others by conventional methods.

1. Collect specimen in a sterile, leakproof container.
2. For respiratory specimens, collect three sputum samples from
deep productive coughs in consecutive 8 to 24 hour intervals per CDC
guidelines (at least one should be an early morning specimen).
3. Place tissue and needle biopsies in Middlebrook broth (available
from Client Supply) or in sterile saline.
4. Culturette and nasopharyngeal swab collections may result in a
potential decline in sensitivity of Mycobacterium isolation in
culture. The yield of material is limited and, due to the
hydrophobicity of Mycobacteria, the organisms may be entrapped
within the fiber matrix and not readily transition into solution or
onto media. False negative cultures are possible, especially if few
pathogens are present.
5. If gastric aspirate cannot be shipped immediately, add 100mg of
sodium bicarbonate as a buffer to neutralize any acid detrimental
to Mycobacteria.
6. Refrigerate all samples within 1 hour of collection time except for
whole blood samples.
7. Ship specimens on cold pack within 24 hours of collection. Whole
blood is shipped at ambient temperature.
Setup Schedule
Sunday-Saturday

Reported (Analytical Time)
52 days


Notes
CPT Codes: 87116; 87206; 87015 1. Source of specimen is required, please include on requisition. 2. Specimens that are heavily contaminated with the normal flora may result in test delay. 3. AFB stain will be performed on whole blood; however, mycobacteria are often present in either very low concentrations or intermittently in peripheral blood. AFB blood stains may therefore yield false negative results. Interpret these results with caution. 4. Notify the laboratory if suspecting Mycobacterium genavense as this organism will not grow on media routinely used for Mycobacterium isolation. 5. The Mycobacterium Tuberculosis Direct (MTD), a target-amplified acid probe is available for respiratory specimens. Call technical services for details. 6. If culture is positive for Mycobacterium species, it will reflex to #2935 AFB Identification by DNA (GenProbe) or #5648 AFB Identification by (HPLC) at an additional charge. 7. If M. tuberculosis complex isolated, susceptibility testing will be performed and a fee will be charged. For other Mycobacterium species, susceptibility testing will be performed upon request.






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For test information, please call Client Services at 800-421-4449.

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