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30264: Shiga Toxins, EIA with Reflex to Escherichia coli O157 Culture Print View
Shiga Toxins, EIA   Not detected   

PRIMARY
1 Stool Cary-Blair Media 1 (1) g   Refrigerated - 4 Day(s)/Room Temperature - 4 Day(s)  
ALTERNATE
  Rectal swab Amies gel   Refrigerated - 48 Hour(s)/Room Temperature - 48 Hour(s)  
  Rectal swab Cary-Blair   Room Temperature - 4 Day(s)/Refrigerated - 4 Day(s)  
  Culture, GN broth   Frozen - 30 Day(s)/Refrigerated - 7 Day(s)  
  Pure Culture Agar Slant   Room Temperature - 7 Day(s)/Refrigerated - 7 Day(s)  

This test detects Shiga toxins produced by enterohemorrhagic E. coli and other enteric organisms which have been isolated from patients who have hemorrhagic colitis with or without hemolytic-uremic syndrome (HUS). Culture allows CDC to track outbreaks by strain typing.

Collect in clean, dry container and then transfer a minimum of 1 gram
or 1 mL into a Cary-Blair stool culture transport medium. Fluid level
should reach line on vial.
Frozen (-70 deg C) specimens are sub-optimal but can be tested
directly without broth enhancement or reflex culture.
Setup Schedule
Sunday-Saturday

Reported (Analytical Time)
3 days

CPT Code
87427

The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.

Notes
Reject Criteria: Swabs not in Amies or Cary-Blair stool culture transport medium; unpreserved raw stool at room temperature; unpreserved raw refrigerated stool >2 hours old from time of collection; frozen specimens in Cary-Blair stool culture transport medium; specimens in Cary-Blair >4 days old; frozen specimens that have been thawed and re-frozen; dry swabs; stool in diapers; unlabeled specimens Methodology: Non-frozen specimens: Broth Amplified Immunoassay with Culture Reflex Frozen specimens: Direct Detection without Amplification or Culture If Shiga Toxins, EIA is "Detected", the E. coli O157 culture will be performed at an additional charge (CPT code(s): 87046).






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