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34257: Herpes Simplex Virus, Type 1 and 2 DNA, Qualitative Real-Time PCR Print View
Source:      
HSV 1 DNA   Not Detected   
HSV 2 DNA   Not Detected   

PRIMARY
1 CSF 1 (0.3) mL   Frozen - 30 Day(s)/Room Temperature - 48 Hour(s)/Refrigerated - 7 Day(s)  
ALTERNATE
  Whole Blood ACD 1 (0.3) mL   Refrigerated - 7 Day(s)/Room Temperature - 48 Hour(s)  
  Bronchial Alveolar Lavage   Frozen - 30 Day(s)/Refrigerated - 7 Day(s)/Room Temperature - 48 Hour(s)  
  Whole Blood EDTA 1 (0.3) mL   Refrigerated - 7 Day(s)/Room Temperature - 48 Hour(s)  
  Bronchial Wash   Frozen - 30 Day(s)/Refrigerated - 7 Day(s)/Room Temperature - 18 Hour(s)  
  APTIMA(R) Unisex Swab Kit   Frozen - 30 Day(s)/Refrigerated - 14 Day(s)/Room Temperature - 14 Day(s)  
  Fluid Pericardial   Frozen - 30 Day(s)/Refrigerated - 7 Day(s)/Room Temperature - 48 Hour(s)  
  Fluid Pleural   Frozen - 30 Day(s)/Refrigerated - 7 Day(s)/Room Temperature - 48 Hour(s)  
  Plasma ACD 1 (0.3) mL   Frozen - 30 Day(s)/Refrigerated - 7 Day(s)/Room Temperature - 48 Hour(s)  
  Plasma EDTA 1 (0.3) mL   Frozen - 30 Day(s)/Room Temperature - 48 Hour(s)/Refrigerated - 7 Day(s)  
  Plasma PPT Tube 1 (0.3) mL   Room Temperature - 48 Hour(s)/Refrigerated - 7 Day(s)/Frozen - 30 Day(s)  
  Serum 1 (0.3) mL   Frozen - 30 Day(s)/Room Temperature - 48 Hour(s)/Refrigerated - 7 Day(s)  
  Amniotic Fluid   Frozen - 30 Day(s)/Room Temperature - 48 Hour(s)/Refrigerated - 7 Day(s)  
  Fluid Vitreous   Refrigerated - 7 Day(s)/Room Temperature - 48 Hour(s)/Frozen - 30 Day(s)  

DNA testing is analytically more sensitive than culture, especially in patients with encephalitis or meningitis. DNA testing may be useful in diagnosis of infection in neonates. Neonates who have been exposed to HSV can develop disseminated infection and encephalitis. Encephalitis is usually due to HSV I whereas meningitis is usually due to HSV II. DNA testing provides reliable means to define the Type.

CSF: Collect in a sterile leak proof container and store refrigerated
or frozen.
Swab: Swab a lesion and place swab in a viral transport media,
VCM, M4, or Aptima(R) Unisex Collection Kit (white label)
Serum: Collect blood in sterile tubes with no anticoagulants; serum
separator tubes (SST(R)) are recommended. Transfer serum into sterile
leak proof container and store refrigerated or frozen.
Bronchial Wash and BAL: Collect in a sterile leak proof
container
Setup Schedule
Monday-Saturday

Reported (Analytical Time)
Same day

CPT Code
87529x2

The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.

Notes
Preferred Specimen:
1 mL (0.3 mL minimum) CSF, pleural fluid, amniotic fluid or pericardial fluid collected in a sterile leak-proof container, serum collected in an SST(R) or sterile tubes with no anticoagulants, whole blood collected EDTA (lavender-top) or ACD (yellow-top) tube, plasma collected in an EDTA (lavender-top) or ACD (yellow-top) or PPT tube. Swab collected in a viral transport media, VCM, M4, or Aptima(R) Unisex Collection Kit (white label) 0.5 mL (0.3 mL minimum) vitreous fluid collected in a sterile leak proof container.
Acceptable Specimen:
1 mL (0.35 mL minimum) Bronchoalveolar lavage or bronchial wash collected in a sterile leak-proof container.
Methodology: Real-Time Polymerase Chain Reaction
Transport Temperature: Refrigerated






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