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35080: Leukemia/Lymphoma Evaluation Print View
Clinical Information:      
Specimen Type:      
Viability: FC    % 
Interpretation: FC     
Sample Description: FC     
Gating Strategy: FC     
Markers: FC     
Number of Markers: FC     

PRIMARY
1 Periph Blood Hep (Green) 5 (3) mL   Room Temperature - 72 Hour(s)  
ALTERNATE
  Bone Marrow EDTA   Room Temperature - 72 Hour(s)  
  Bone Marrow Heparinized   Room Temperature - 72 Hour(s)  
  Tissue   Refrigerated - 72 Hour(s)  
  Periph Blood ACD   Room Temperature - 72 Hour(s)  
  Periph Blood EDTA   Room Temperature - 72 Hour(s)  
  Bone Marrow ACD Sol A   Room Temperature - 72 Hour(s)  
  Fluid   Refrigerated - 72 Hour(s)  

To aid in the diagnosis of leukemia or lymphoma. Initial markers evaluated: CD2, CD3, CD4, CD5, CD7, CD8, CD10, CD11c, CD13, CD19, CD20, CD23, CD33, CD34, CD38, CD56, CD64, CD117, HLA-DR, sKappa, sLambda. CD 45 is used for gating.

Peripheral blood and bone marrow:
The tube must be kept at room temperature and shipped to the lab
immediately.
Tissue:
Any tissue type is acceptable. Tissue size is dependent upon leukocyte
cellularity. (The tissue is disaggregated into single cells so
that a minimum of 50,000 cells of interest are harvested.). Ship
tissue in sterile plastic container with RPMI 1640 enriched with
FBS (10% FBS RPMI). Absolutely no fixative should be added.
Refrigerate and ship immediately.
Body Fluids:
Any body fluid is acceptable. Sample size is dependent
upon cellularity of the sample. (A minimum of 50,000 cells of interest
in total volume of fluid). Place fluid in sterile plastic container.
Absolutely no fixative should be added. Refrigerate and ship
immediately.

A clinical indication and specimen source are required with each
specimen. If possible, submit CBC results with differential or an EDTA
tube of peripheral blood.
Do not freeze and do not place in fixative.
Because of the critical nature of these specimens, the laboratory will
attempt to process all specimens received, regardless of age of
specimen.
Setup Schedule
Sunday-Saturday

Reported (Analytical Time)
Next day


Notes
**This test is not available for New York patient testing.**
Includes: CD2, CD3, CD5, CD4, CD7, CD8, CD10, CD11c, CD13, CD19, CD20, CD23, CD33, CD34, CD38, CD56, CD64, CD117, HLADR, Kappa, Lambda, CD45.
Transport Temperature:
Peripheral Blood and Bone Marrow: Room temperature
Tissue and Body fluids: Refrigerated
Specimen Stability:
Room temperature: 72 hours (may extend to 5 days depending on cell viability).
Refrigerated and Frozen: Not established
Specimen Requirements:
Preferred:
5 mL (3 mL minimum) peripheral blood collected in a sodium heparin (green-top), ACD-A (yellow-top), or EDTA (lavender-top) tube OR 4 mL (1 mL minimum) bone marrow collected in a sodium heparin (green-top), ACD-A (yellow-top), or EDTA (lavender-top) tube OR
Tissue OR Body Fluids: Any type is acceptable
Acceptable:
Bone Marrow: Pediatric heparin tube
Reject Criteria: See instructions
Assay Category: LDT/ASR Class 1
Additional Information:
*Additional markers may be performed based on the pathologist review. These markers will be performed at an additional charge (CPT code(s):
88185 for each additional marker).






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