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36586: Histamine, Plasma Print View
Histamine, Plasma EIA    ng/mL 

1 Plasma EDTA 1 (0.5) mL   Room Temperature - 24 Hour(s)/Refrigerated - 24 Hour(s)/Frozen - 7 Day(s)  

In humans, histamine (beta-imidazole ethylamine) is an important mediator of allergic responses and is mostly found in the initial phase of an anaphylactic reaction ("immediate type" allergy). Histamine is developed by the enzymatic decarboxylation of histidine. In the organism, histamine is present in nearly all tissues, and is mainly stored in the metachromic granula of mast cells and the basophilic leukocytes. It is present in an inactive bound form and is only released as required. Histamine acts predominantly on smooth muscle and blood vessels. In humans, it is responsible for the bronchoconstriction occurring during the acute phase. In the vessels, its constrictive effect is limited to the venula, whereas arterioles are dilated. Furthermore, histamine causes a contraction of the cells of the vascular permeability, thereby allowing higher-molecular substances to escape into the tissue. Like several other mediators, histamine does not only mediate various clinical symptoms of anaphylactic reaction. Histamine may modulate immune mediated responses. The biological action of histamine in tissue is guaranteed by three different surface receptors, i.e., H1, H2, and H3 receptors. Histamine is released from mast cells and basophils following allergen cross linking of Fc epsilon receptor I through the allergen's interaction with receptor bound allergen specific IgE. Immediate hypersensitivity reactions are mediated via histamine released by mast cells. Histamine release by basophils serves to prolong and amplify hypersensitivity reactions. Severe mast cell degranulation may be associated with anaphylaxis.

Centrifuge immediately and freeze in plastic screw-cap vial.

Patient Preparation:
Avoid taking allergy causing drugs, antihistamines, oral
corticosteroids and substances which block H2 receptors 24
hours prior to collection.
Setup Schedule
Tuesday, Friday

Reported (Analytical Time)
Same day

CPT Code

The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.

Preferred Specimen: 1 mL Plasma EDTA (lavender-top)
Transport Temperature: Frozen
Reject Criteria: Hemolysis; Lipemia; Received room temperature; Received refrigerated
Methodology: Enzyme Immunoassay

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