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37273: Hepatitis C Viral RNA, Qualitative TMA Print View
HCV RNA, QL, TMA TMA  Not detected   

PRIMARY
1 Plasma PPT Tube 2 (0.6) mL   Frozen - 42 Day(s)/Refrigerated - 14 Day(s)/Room Temperature - 72 Hour(s)  
ALTERNATE
  Plasma ACD 2 (0.6) mL   Frozen - 42 Day(s)/Refrigerated - 14 Day(s)/Room Temperature - 72 Hour(s)  
  Serum 2 (0.6) mL   Room Temperature - 72 Hour(s)/Refrigerated - 14 Day(s)/Frozen - 42 Day(s)  
  Plasma EDTA 2 (0.6) mL   Frozen - 42 Day(s)/Refrigerated - 14 Day(s)/Room Temperature - 72 Hour(s)  
  Sodium Heparin   Room Temperature - 72 Hour(s)/Refrigerated - 14 Day(s)/Frozen - 42 Day(s)  
  Plasma Citrated 2 (0.6) mL   Frozen - 42 Day(s)/Refrigerated - 14 Day(s)/Room Temperature - 72 Hour(s)  

Qualitative RNA by TMA is useful in confirming HCV infection and to assess response to therapy.

Plasma: Collect blood in sterile tubes containing EDTA anticoagulant;
either 0.15% solution v/v final EDTA K3 (standard EDTA tube) or 9 mg
spray-dried EDTA K2 (Plasma Preparation Tube or PPT tube with plasma
separator-gel, preferred). Blood collected in tubes containing ACD
anticoagulant are acceptable but will yield results approximately
15% lower when compared to EDTA tubes due to the dilution effect of
the 1.5 mL of anticoagulant used in the tube. Store whole blood at
room temperature and separate plasma from cells within 6 hours of
collection. Transfer plasma to sterile, plastic, screw-capped aliquot
tubes and store at -18 C or colder. Do not clarify plasma by
filtration or further centrifugation. Avoid repeated freezing and
thawing of specimen.
Note: If blood is collected in a PPT tube, centrifuge within 6 hours
of collection as before, but it is not necessary to transfer the
plasma to aliquot tubes. Following centrifugation, a gel barrier
maintains separation of plasma from cellular components during
specimen transport and store, and unlike standard VACUTAINER Brand
blood collection tubes, the PPT tube is plastic and hence the plasma
can be shipped and stored frozen in the original tube.
Serum: Collect blood in sterile tubes with no anticoagulants; serum
separator tubes (SSTs) are recommended. Allow blood to clot at room
temperature and separate serum from cells within 6 hours of
collection. Transfer serum to sterile, plastic screw-capped, aliquot
tubes and store at -18 C or colder. Avoid repeated freezing and
thawing of specimen.
Setup Schedule
Tuesday-Saturday

Reported (Analytical Time)
5 days

CPT Code
87521

The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.

Notes
Methodology: Transcription-mediated Amplification Reject Criteria: Unspun PPT tube






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