This assay measures the capability of neutrophils (PMN) to undergo oxidative metabolism to produce superoxide anion and hydrogen peroxide, as detected by oxidation of dihydrorhodamine (DHR). Patients with Chronic Granulomatous Disease (CGD) are unable to oxidize DHR due to defects in oxidative metabolism. The proportion of neutrophils with intact oxidative capacity slowly decreases as the specimen age (hours post-collection) increases. It is thus important that the reference range value appropriate for the specimen age be utilized when assessing the reported results.
Specimen must arrive at the referral laboratory within 72 hours
of collection in order to meet sample stability requirements.
Specimens are accepted <72 hours post collection. No other
perservatives are acceptable.
Specify time of day specimen was collected.
Clients may send 4 mL whole blood in sodium heparin from a healthy
donor unrelated to the patient, drawn at the same time as the
patient sample to run in parallel with the patient's specimen. The
outermost label of the tube must be clearly labeled as
"Transport Control". If the draw date is not indicated on the
specimen, the client must be called and this information obtained.
Clients: Contact the lab prior to ordering for special logistics
PSC: Follow Short Stability Protocol.
Test available by prior arrangement only. Special sample collection
and transportation arrangements must be made prior to ordering the
test. Contact your local Customer Service Department and request to
speak to someone in Referral Testing Department for specific
Submit Monday - Thursday only. Do not draw day before a
holiday. Draw the specimens prior to the last FedEx pick up. Make
arrangements with FedEx in advance for same day pick up. Collect and
label the specimen(s) according to test requirements. Place the
specimen(s) and paperwork into the short stability specimen kit. Use
one kit per patient. Use the pre-printed FedEx shipping airbill
(provided with the kit) for FedEx Express overnight shipment to Quest
Diagnostics Nichols Institute in San Juan Capistrano, CA.
Request item #170903
Samples received > 72 hours after collection will be rejected. Date
and time of draw are required and must be provided.
Reported (Analytical Time)
The CPT codes provided are based on AMA guidelines and are for informational purposes only.
CPT coding is the sole responsibility of the billing party.
Please direct any questions regarding coding to the payer being billed.