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4469: Culture, Anaerobic Bacteria With Gram Stain Print View
Specimen Source:      
Gram Stain:      
Anaerobic Culture      

Anaerobic bacteria cause a variety of human infections. The results of anaerobic cultures assist the clincian with diagnosis and treatment of patients with anaerobic infections.

Specimen Requirements:
3mL or 3 grams (1 mL or 1 g minimum) abscess or Deep Wound or Tissue
or Normally sterile body fluids other than blood or
Bronchoscopic specimens obtained by the Double Lumen Brush technique,
or Suprapubic urine aspirates obtained from bladder or Nephrostomy
tube or suprapubic catheterization or Aspirated pus from deep wound or
abscess or tympanocentesis fluid or Genital specimens
from these sites only:
-cul-de-sac aspiration-culdocentesis-percutaneous transfundal
aspiration or Genital specimens from these sites only:
fallopian tube, surgical specimens, exudates collected in
Anaerobic transport gel vial Swabs in Amies gel (blue cap)
transport medium eSwab (white-cap).

Acceptable: (2 swabs) abscess or deep wound, normally sterile body
fluids other than blood, bronchoscopic specimens obtained by the
Double Lumen Brush techinique, suprapubic urine aspirates obtained
from bladder, nephrostomy tube or suprapubic catheterization,
aspirated pus from deep wound or abscess or tympanocentesis fluid,
genital specimens from these sites only:-cul-de-sac aspiration-
culdocentesis-percutaneous transfundal aspiration, fallopian tube,
surgical specimens, exudates collected in Amies gel (blue-cap) or
ESwab (white-cap)

Tissues and fluids are superior to a swab specimen. If swabs must
be used, collect 2 anaerobic transport swabs, one for culture and
one for gram stain OR submit one anaerobic transport swab with one
air dried smear OR one ESwab (white-cap) for both culture and gram
stain. Specimens not listed as acceptable may be processed for
anaerobic culture only after consultation with a Quest Microbiology
Scientific Director. Do not submit syringe with needle attached.
If submitting syringe, remove needle, expel air and cap syringe.
Purulent sterile body fluids not submitted in an anaerobic transport
tube may be accepted for culture if received within 8 hours of

Transport Te
Setup Schedule

Reported (Analytical Time)
5 days

CPT Codes

The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.

Reject Criteria: Specimens not in anaerobic transport medium; expired transport media; throat, nasopharyngeal, sputum or bronchoscopic specimens; specimens not collected by Double Lumen Brush technique; stool and rectal swabs; urine-voided or catherer; vaginal and cervical; specimens from sites contaminated with intestinal contents; superficial or fresh wounds; external ear; external eye; intrauterine device; tracheal aspirates
Methodology: Direct Microscopy; Bacterial Culture; Anaerobic Isolation and Identification Procedures; Phenotypic; Matrix Assisted Laser Desorption Ionization-Time of Flight; Sequencing If culture is positive, organism identifications will be performed at an additional charge (CPT codes(s):87076, 87077). Antibiotic susceptibility testing is only performed when appropriate (CPT codes(s): 87181 (per antimicrobial agent), or 87186). If the organism(s) cannot be identified by the original method, additional charges (CPT codes(s): DNA sequencing 87153, Serological Typing 87147) may be added.

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