Preferred Specimen: 10 mL (2 mL minimum) body fluid or lower
respiratory tract specimens OR 10 mL (5 mL minimum) gastric lavage
OR 2 mL (2 mL minimum) CSF OR 2 grams (1 gram minimum) fresh
(unfixed) tissue OR 50 mL (40 mL minimum) urine collected in a
sterile, leak-proof container OR 1 mL or 1 swab wound, exudates,
aspirates, lesion material, ocular specimens collected in a sterile,
leak-proof container or Amies liquid, Amies gel swab transport
system, Stuarts or ESWAB.
Tissue and biopsy material: Sterile, leak-proof container with small
amount of saline, no fixative or preservative.
Gastric lavage fluid: Sterile, leak-proof container. Must be
neutralized with sodium bicarbonate within 4 hours of collection.
Expectorated sputum: Instruct patient to gargle with water and
cough deeply. Three separate early morning collections on at least
three consecutive days are recommended.
Reject Criteria: Specimen in formalin or alcohol; leaked specimen;
24-hour sputum or urine; urine in preservative.
Reported (Analytical Time)
Transport Temperature: Refrigerated Methodology: Continuous monitoring broth culture system & conventional culture, Microscopic Exam with Flurochrome Stain.