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4554: Mycobacteria, Culture, With Fluorochrome Smear Print View
Source      
Acid-fast Bacilli Stain STAIN  No AFB Seen   
Preliminary Culture CULTURE     
Preliminary Culture CULTURE     

PRIMARY
1 CSF 2 (2) mL   Refrigerated - 5 Day(s)  
2 Culturette/Swab 1 (1) mL   Refrigerated - 5 Day(s)  
3 Fluid Ascitic 10 (2) mL   Refrigerated - 5 Day(s)  
4 Fluid 10 (2) mL   Refrigerated - 5 Day(s)  
5 Fluid Pericardial 10 (5) mL   Refrigerated - 5 Day(s)  
6 Fluid Peritoneal 10 (2) mL   Refrigerated - 5 Day(s)  
7 Urine 50 (40) mL   Refrigerated - 5 Day(s)  
8 Fluid Synovial 10 (2) mL   Refrigerated - 5 Day(s)  
9 Gastric Aspirate 1 (1) mL   Refrigerated - 5 Day(s)  
10 Nasopharyngeal Swab 1 (1) mL   Refrigerated - 5 Day(s)  
11 Sterile Container/Tube 2 (1) mL   Refrigerated - 5 Day(s)  
12 Fluid Pleural 10 (2) mL   Refrigerated - 5 Day(s)  
13 Tissue 2 (1) g   Refrigerated - 5 Day(s)  
ALTERNATE
  Stool   Refrigerated - 5 Day(s)  

Preferred Specimen: 10 mL (2 mL minimum) body fluid or lower
respiratory tract specimens OR 10 mL (5 mL minimum) gastric lavage
OR 2 mL (2 mL minimum) CSF OR 2 grams (1 gram minimum) fresh
(unfixed) tissue OR 50 mL (40 mL minimum) urine collected in a
sterile, leak-proof container OR 1 mL or 1 swab wound, exudates,
aspirates, lesion material, ocular specimens collected in a sterile,
leak-proof container or Amies liquid, Amies gel swab transport
system, Stuarts or ESWAB.

Tissue and biopsy material: Sterile, leak-proof container with small
amount of saline, no fixative or preservative.
Gastric lavage fluid: Sterile, leak-proof container. Must be
neutralized with sodium bicarbonate within 4 hours of collection.

Patient Preperation:

Expectorated sputum: Instruct patient to gargle with water and
cough deeply. Three separate early morning collections on at least
three consecutive days are recommended.

Reject Criteria: Specimen in formalin or alcohol; leaked specimen;
24-hour sputum or urine; urine in preservative.
Setup Schedule
Sunday-Saturday

Reported (Analytical Time)
52 days


Notes
Transport Temperature: Refrigerated

Methodology: Continuous monitoring broth culture system & conventional culture, Microscopic Exam with Flurochrome Stain.






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