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90479: RAS Mutation Analysis, Thyroid Cancer [90479] Print View
Specimen Source      
Block/Specimen ID      
HRAS Mutation   Not detected   
KRAS Mutation   Not detected   
NRAS Mutation   Not detected   

PRIMARY
1 Slides (Unstained) 4 (4) mL   Room Temperature - 12 Month(s)/Refrigerated - 12 Month(s)  
ALTERNATE
  Tissue Paraffin Block   Refrigerated - 12 Month(s)/Room Temperature - 12 Month(s)  

Activating mutations in the RAS genes, particularly NRAS, have been detected in follicular thyroid carcinomas (up to 40%) and papillary thyroid carcinomas (up to 10%), but are also seen commonly in follicular adenomas. Correlation with morphologic and clinical findings is thus required. Follicular thyroid neoplasms with RAS mutations may behave more aggresively than those without. The assay tests for mutations in codon 12 and 13 (exon 1) and codon 61 (exon 2) in HRAS, KRAS and NRAS by a DNA-based sequencing method, with an approximate sensitivity of 1 to 5% mutation-bearing cells in a mixed sample. This assay will not detect RAS mutations outside the codons listed above.

Needle washings in alcohol based fixative (e.g. cytolyt), 4 slides, or
Formalin fixed, paraffin embedded tissue block FNA or FFPE specimens
are acceptable for the assay. All specimens must be accompanied with
Pathology report. Specimens will be examined by a pathologist for
confirmation and microdissection if warranted. A minimum of 4 FNA
slides should be submitted and three should be without coverslips.
Coverslipped slides will delay reporting of results.

Transport Temperature: Room temperature
Setup Schedule
Tuesday, Thursday

Reported (Analytical Time)
3 days


Notes
METHODOLOGY: Pyrosequencing






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