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90879: Factor VIII Antigen Print View
Factor VIII Antigen   0.6 - 1.95  IU/mL 

PRIMARY
1 Plasma Citrated 1 (0.5) mL   Room Temperature - 4 Hour(s)/Refrigerated - 48 Hour(s)/Frozen - 30 Day(s)  
ALTERNATE

The laboratory diagnosis of Factor VIII deficiency typically involves quantitative determinations based on procoagulant levels (functional activity of Factor VIII typically measured by clotting assay). Clinical bleeding symptoms may also be used in the diagnosis and classification, however, the quantitative procoagulant measurement is the preferred method of classifying the severity of hemophilia. An ELISA for Factor VIII antigen may be used in conjunction with the functional assays in the assessment of Factor VIII replacement therapies, assessment of carrier status as well as to distinguish those patients that may be deemed CRM-Positive.

Platelet-poor plasma: Centrifuge light blue-top tube for 15
minutes at approximately 1500 g within 60 minutes of collection.
Using a plastic pipette, remove plasma, taking care to avoid the
WBC/platelet buffy layer and place into a plastic vial.
Centerifuge a second time and transfer platelet-poor plasma into a
new plastic vial. Plasma must be free of platelets (<10,000/mcL).
Freeze immediately and ship on dry ice.
Setup Schedule
Wednesday

Reported (Analytical Time)
2-31 days

CPT Code
85244

The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.

Notes
METHODOLOGY: Enzyme Linked Immunosorbent Immunoassay Transport Temperature: Frozen/Do not thaw Shipping room temperature unacceptable; Shipping refrigerated unacceptable Specimen Stability: Room temperature: 4 Hours; Refrigerated: 48 Hours; Frozen: 30 Days; -70 Degrees: 6 Months Setup Schedule: Set up as needed at least 1X a month: Report available: 2-31 Days






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