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91299: HIV-1 Coreceptor Tropism, Proviral DNA Print View
CXCR4(X4)      
Net Tropism Assessment      
MVC Activity Anticipated      

PRIMARY
1 K2 EDTA 2 (0.6) mL   Room Temperature - 7 Day(s)/Refrigerated - 7 Day(s)/Frozen - 28 Day(s)  
ALTERNATE
  Periph Blood Mononuc Cell   Frozen - 28 Day(s)  

The use of CCR5 antagonists requires screening for viral tropism to exclude patients harboring X4 or D/M virus. HIV-1 Proviral DNA tropism test is useful for determination of viral tropism in patients with undetectable HIV-1 viral RNA (virologically suppressed).

BLOOD: collect blood in K2 EDTA (lavender-top) tube.
PERIPHERAL BLOOD MONONUCLEAR CELLS: collect blood in BD Vacutainer(R)
CPT(TM) tube. After collection, store tube upright at room temperature
for up to two hours before centrifugation. Remix blood sample
immediately prior to centrifugation by inverting 10 times. Centrifuge
at 800g for 30 min, room temperature with brake off. Immediately
remove plasma (>=3mL) from top layer without disturbing cell layer.
Add 0.5 mL phosphate-buffered saline to remaining layer of
plasma/cells. Cap tube and gently mix by inversion 10 times. Transfer
the upper layer of cells (up to 3 mL) in plasma/phosphate-buffered
saline to a sterile K2 EDTA tube. Store and ship frozen at <-60
degrees Celsius. DO NOT send original glass CPT(TM) or plasma
supernatant to the lab.
Setup Schedule
Tuesday, Friday

Reported (Analytical Time)
4 days

CPT Code
87906

The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.

Notes
Method: Polymerase Chain Reaction (PCR) and DNA Sequencing






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