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91976: Mitogen-induced Lymphocyte Proliferation Panel (PHA, Con A, PWM) Print View
PHA, CPM   Male: 73700 - 265000
Female: 53000 - 200000 
Net CPM 
PHA, SI     SI 
Con A, CPM   Male: 46100 - 283000
Female: 49500 - 129000 
Net CPM 
Con A, SI     SI 
PWM, CPM   29100 - 125000  Net CPM 
PWM, SI     SI 
Cell Viability   80 - 100  % 

PRIMARY
1 Whole Blood Heparin 10 (5) mL   Room Temperature - 48 Hour(s)  
ALTERNATE

Measurement of human lymphocytes' proliferative responses to various stimuli is a fundamental technique used to assess their biological status and functions. Mitogens, such as plant lectins phytohemagglutinin (PHA), concanavalin A (Con A) and pokeweed mitogen (PWM), are able to nonspecifically stimulate lymphocyte proliferation and used to evaluate patient immune responsiveness. Both T and B lympocytes are stimulated by PWM, while only T cells are stimulated by PHA and Con A. Impaired responses to these mitogens are seen in immunodeficiency diseases, certain infections, and cancers. Lymphocyte proliferative response to antigens, such as candida, tetanus toxoid, and tuberculin purified protein derivative (PPD) are evaluated as a function of memory in cell-mediated immunity. Lack of response can indicate either the absence of prior exposure to specifics antigens, a waning response to the antigen following exposure many years earlier, or a general decrease in lymphocyte reactivity due to primary or secondary immune deficiency. Note that even in healty individuals, lymphocyte proliferative responses are known to be affected by the use of certain medications, alcohol, and stress.

Whole blood must be transported at room temperature and delivered to
the testing laboratory within 48 hours after collection.
Maintain and transport blood at room temperature. Avoid temperatures
<15 degrees C and >37 degrees C. In hot weather, it may be necessary
to pack the specimen in a container with insulating material around
it and place this container inside another one that contains a cold
pack (ice pack) and absorbent material. This method will help retain
the specimen at ambient temperature.
For longitudinal studies, draw specimens at the same time of day to
minimize diurnal variation.

CLIENTS: Contact the lab prior to ordering for special logistics
arrangements.
PSC: Follow Short Stability Protocol.

Test available by prior arrangement only. Special sample collection
and transportation arrangements must be made prior to ordering the
test. Contact your local Customer Service Department and request to
speak to someone in Referral Testing Department for specific
instructions.
Submit Monday - Thursday only. Do not draw day before a holiday.
Samples received in testing lab >48 hours after collection will be
rejected. Date and time of draw are required and must be provided.
Setup Schedule
Tuesday-Friday

Reported (Analytical Time)
14 days

CPT Code
86353x3

The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.

Notes
Reject Criteria: Hemolysis; received refrigerated; received frozen; clotted specimens; samples collected in lithium heparin tubes METHODOLOGY: Cell culture and scintillation counter






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