Measurement of human lymphocytes' proliferative responses to various stimuli is a fundamental technique used to assess their biological status and functions. Mitogens, such as plant lectins phytohemagglutinin (PHA), concanavalin A (Con A) and pokeweed mitogen (PWM), are able to nonspecifically stimulate lymphocyte proliferation and used to evaluate patient immune responsiveness.
Whole blood must be transported at room temperature and delivered to
the testing laboratory within 48 hours after collection.
Maintain and transport blood at room temperature. Avoid temperatures
<15 degrees C and >37 degrees C. In hot weather, it may be necessary
to pack the specimen in a container with insulating material around
it and place this container inside another one that contains a cold
pack (ice pack) and absorbent material. This method will help retain
the specimen at ambient temperature.
For longitudinal studies, draw specimens at the same time of day to
minimize diurnal variation.
CLIENTS: Contact the lab prior to ordering for special logistics
PSC: Follow Short Stability Protocol.
Test available by prior arrangement only. Special sample collection
and transportation arrangements must be made prior to ordering the
test. Contact your local Customer Service Department and request to
speak to someone in Referral Testing Department for specific
instructions. Submit Monday - Thursday only. Do not draw day before a
holiday. Samples received in testing lab >48 hours after collection
will be rejected. Date and time of draw are required and must be
Reported (Analytical Time)
The CPT codes provided are based on AMA guidelines and are for informational purposes only.
CPT coding is the sole responsibility of the billing party.
Please direct any questions regarding coding to the payer being billed.
Reject Criteria: Hemolysis; received refrigerated; received frozen; clotted specimens; samples collected in lithium heparin tubes
METHODOLOGY: Cell culture and scintillation counter