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93870: Zika Virus RNA Qualitative Real-Time RT-PCR Print View
Zika RNA Real-Time RT-PCR RT-PCR     
Pregnant (Y/N)      
Travel in Zika Region?      
Resident of Zika Region?      
Clinical Signs/Symptoms?      
Date of Onset (mm/dd/yy)      

1 Serum 3 (1) mL   Refrigerated - 7 Day(s)/Frozen - 30 Day(s)  
  Serum Separator Tube 3 (1) mL   Refrigerated - 7 Day(s)/Frozen - 30 Day(s)  

Testing should only be performed on individuals meeting Centers for Disease Control and Prevention (CDC) Zika virus clinical criteria (e.g., a history of clinical signs and symptoms associated with Zika virus infection); CDC Zika virus epidemiological criteria (e.g., history of residence in or travel to a geographic region with active Zika transmission at the time of travel, or other epidemiologic criteria for which Zika virus testing may be indicated); and/or the CDC guidelines. Most people with Zika virus infection are asymptomatic. Symptomatic individuals typically experience a mild illness characterized by fever, joint pain, rash, or conjunctivitis. Clinical illness is usually self-limited and lasts a week or less. Not all symptomatic patients report all of these clinical findings, and Zika manifestations overlap significantly with those seen in other viral infections. The incubation period is unclear, but likely to be several days. Symptoms generally resolve on their own within a week. Pregnant women at risk for Zika virus in the U.S. and in endemic areas are of special concern due to the increased incidence of babies with microcephaly or other congenital defects if mothers are infected during pregnancy. Please refer to the CDC guidelines for the appropriate testing in pregnant women ( Zika virus RNA is generally detectable in serum and/or urine during the acute phase of infection and up to 14 days following onset of symptoms, if present. Zika virus RNA may be detected longer in a pregnant woman. Thus, the optimum time to perform serum and urine RNA testing is during the first two weeks (<14 days) after the onset of clinical illness in non-pregnant symptomatic patients. During pregnancy, the duration of viremia and/or viruria may be prolonged. Evidence suggest that pregnant woman may have detectable virus in serum and urine for up to 14 d

* Collect blood in a serum separator tube (preferred) or a standard
* Allow blood to clot at room temperature.
* Blood collection tube should be centrifuged as soon as possible and
serum transferred to a plastic transfer tube in order to avoid
* Transport refrigerated (cold packs) to local Quest Diagnostics
accessioning laboratory. IMPORTANT: If sample is being shipped
directly to the performing laboratory facility by an overnight
air courier, then transport it frozen on dry ice.
* Specimens must not be left in lock boxes.
Setup Schedule

Reported (Analytical Time)
Next day

CPT Code

The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.

Methodology: Real-Time Reverse Transcriptase Polymerase Chain Reaction

Specimen Stability:
-20 degrees C Unacceptable -70 degrees C 30 days

Transport Temperature: Refigerated

Reject Criteria:
Heparinized specimens; serum recieved in a frozen serum separator tube (SST); serum received in an unspun serum separator tube (SST); serum received in a standard red-top tube (not transferred to a plastic transfer tube)

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For test information, please call Client Services at 800-421-4449.

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