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93870: Zika Virus RNA Qualitative Real-Time RT-PCR Print View
Zika RNA Real-Time RT-PCR RT-PCR     
Pregnant (Y/N)      
Travel in Zika Region?      
Resident of Zika Region?      
Clinical Signs/Symptoms?      
Date of Onset (mm/dd/yy)      

PRIMARY
1 Serum 3 (0.6) mL   Room Temperature - 4 Hour(s)/Refrigerated - 7 Day(s)/Frozen - 30 Day(s)  
ALTERNATE
  Serum Separator Tube 3 (0.6) mL   Room Temperature - 4 Hour(s)/Refrigerated - 11 Day(s)/Frozen - 30 Day(s)  

Testing should only be performed on individuals meeting Centers for Disease Control and Prevention (CDC) Zika virus clinical criteria (e.g., clinical signs and symptoms associated with Zika virus infection) and/or CDC Zika virus epidemiological criteria (e.g. history of residence in, or travel to a geographic region with active Zika transmission at the time of travel, or other epidemiologic criteria for which Zika virus testing may be indicated, such as possible sexual transmission). Most people with Zika virus infection are asymptomatic. Symptomatic individuals typically experience a mild illness characterized by fever, joint pain, rash, or conjunctivitis. Clinical illness is usually self- limited and lasts a week or less. Not all symptomatic patients report all of these clinical findings, and Zika manifestations overlap significantly with those seen in other viral infections. The incubation period is unclear, but likely to be several days. Symptoms generally resolve on their own within a week. Pregnant women at risk for Zika virus in the U.S. and in endemic areas are of special concern due to the increased incidence of babies with microcephaly or other congenital defects if mothers are infected during pregnancy. Please refer to the CDC guidelines for the appropriate testing in pregnant women (http://www.cdc.gov/zika/hc-providers/pregnant-woman.html). Zika virus RNA may be detected in serum for approximately 4-7 days following onset of symptoms; but may be detected longer in a pregnant woman, thus the optimum time to perform serum RNA testing is during the first week after the onset of clinical illness in non-pregnant patients. During pregnancy, the duration of viremia and/or viruria may be prolonged. Evidence suggests that pregnant women may have detectable virus in serum for up to 14 days or longer, therefore PCR testing is recommended at least up to 14 days or longer in a pregnant woman. For pat

Order Zika test separately from other tests - on a separate
test requisition. Collect blood in a serum separator vacutainer
tube (preferred) or a standard red-top. Allow blood to clot
at room temperature.
Blood collection tube should be centrifuged as soon as possible
and serum transferred to a plastic tube, in order to avoid hemolysis.

Mark as "Irreplaceable Specimen" and follow Irreplaceable Specimen
procedures.
Transport refrigerated (cold packs) to local Quest Diagnostics
accessioning laboratory.
Specimens must not be left in lock boxes.
The local Quest laboratory will ship frozen (dry ice) to the
performing laboratory, if different than the local Quest laboratory.
Alternatively for hospitals that ship directly to the performing
laboratory ship serum frozen (dry ice). (Samples are stable
refrigerated for up to 7 days before shipping on dry ice.)
Avoid freeze-thaw cycles.
Setup Schedule
Monday-Saturday

Reported (Analytical Time)
Next day

CPT Code
87798

The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.

Notes
This test is only for use under the Food and Drug Administration's (FDA) Emergency Use Authorization. Methodology: Real-Time Reverse Transcriptase Polymerase Chain Reaction Specimen Stability: -20 degrees C Unacceptable -70 degrees C 30 days Transport Temperature: -70 degrees Celsius Reject Criteria: Serum received in a frozen serum separator tube Serum received in an unspun serum separator tube Acceptable Specimen: Seum separator tube, received spun






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