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94221: Zika Virus RNA, Qualitative Real-Time RT-PCR Panel, Serum/Urine Print View
Zika RNA, Serum RT-PCR     
Zika RNA, Urine RT-PCR     
Pregnant? (Y/N)      
Travel in Zika Region?      
Resident of Zika Region?      
Clinical Signs/Symptoms?      
Date of Onset (MM/DD/YY)      

PRIMARY
1 Serum 3 (0.6) mL   Refrigerated - 7 Day(s)  
2 Urine 3 (0.6) mL   Refrigerated - 7 Day(s)  
ALTERNATE
  Serum Separator Tube 3 (0.6) mL   Refrigerated - 7 Day(s)  

Testing should only be performed on individuals meeting Centers for Disease Control and Prevention (CDC) Zika virus clinical criteria (e.g., clinical signs and symptoms associated with Zika virus infection) and/or CDC Zika virus epidemiological criteria (e.g. history of residence in, or travel to a geographic region with active Zika transmission at the time of travel, or other epidemiologic criteria for which Zika virus testing may be indicated, such as possible sexual transmission). Most people with Zika virus infection are asymptomatic. Symptomatic individuals typically experience a mild illness characterized by fever, joint pain, rash, or conjunctivitis. Clinical illness is usually self-limited and lasts a week or less. Not all symptomatic patients report all of these clinical findings, and Zika manifestations overlap significantly with those seen in other viral infections. The incubation period is unclear, but likely to be several days. Symptoms generally resolve on their own within a week. Pregnant women at risk for Zika virus in the U.S. and in endemic areas are of special concern due to the increased incidence of babies with microcephaly or other congenital defects if mothers are infected during pregnancy. Please refer to the CDC guidelines for the appropriate testing in pregnant women (http://www.cdc.gov/zika/hc-providers/pregnant-woman.html). Zika virus RNA may be detected in serum for approximately 4-7 days following onset of symptoms; but may be detected longer in a pregnant woman, thus the optimum time to perform serum RNA testing is during the first week after the onset of clinical illness in non-pregnant patients. During pregnancy, the duration of viremia and/or viruria may be prolonged. Evidence suggests that pregnant women may have detectable virus in serum for up to 14 days or longer, therefore PCR testing is recommended at least up to 14 days or longer in a pregnant woman. Optima

Order Zika tests separately from other tests - on a separate
requisition.

Collect blood in a serum separator vacutainer tube (preferred) or a
standard red-top.
Allow blood to clot at room temperature.
Blood collection tube should be centrifuged as soon as possible and
serum transferred to a plastic tube, in order to avoid hemolysis.

Urine should be collected in a sterile container without additives.
Urine should always be collected with a patient-matched serum
specimen. Immediately refrigerate.

Mark as "Irreplaceable Specimen" and follow Irreplaceable Specimen
procedures.

Transport refrigerated (cold packs) to local Quest Diagnostics
accessioning laboratory.
Specimens must not be left in lock boxes.
Setup Schedule
Monday-Saturday

Reported (Analytical Time)
Next day

CPT Code
87798x2

The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.

Notes
Specimen Stability: Room temperature, Frozen and -70 degrees Celsius: Unacceptable The test is only for use under the Food and Drug Administration's Emergency Use Authorization. Methodology: Real-Time Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) Transport Temperture: Refrigerated Reject Criteria: Serum received in a frozen serum separator tube Serum received in an unspun serum separator tube Acceptable Specimen: Serum separator tube, received spun






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